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- Title
Mannose-binding lectin gene polymorphism in relation to periodontal infection.
- Authors
Liukkonen, A.; He, Q.; Gürsoy, U. K.; Pussinen, P. J.; Gröndahl ‐ Yli ‐ Hannuksela, K.; Liukkonen, J.; Sorsa, T.; Suominen, A. L.; Huumonen, S.; Könönen, E.
- Abstract
Background and Objective Mannose-binding lectin ( MBL) plays an important role in innate immunity. MBL deficiency is usually caused by mutations in exon 1 of the MBL structural gene ( MBL2). Our aim was to investigate MBL2 polymorphisms and their relation to salivary levels of periodontal inflammatory/tissue destruction markers and two major periodontitis-associated bacteria. Material and Methods Salivary samples from 222 subjects were available for genotyping by pyrosequencing. The subjects between 40 and 60 years of age and having a minimum of 20 teeth were divided into three periodontal groups: 80 had generalized periodontitis, 65 had localized periodontitis and 77 were periodontitis-free. A comparison between their MBL2 genotypes and salivary detection rates and levels of Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis as well as interleukin -1β, matrix metalloproteinase -8, and tissue inhibitor of matrix metalloproteinase ( TIMP)-1 was performed. Results The frequencies of the MBL2 wild-type (A/A), heterozygote variants (A/O) and homozygote variants (O/O) were 69.4%, 26.6% and 4%, respectively. In A. actinomycetemcomitans-positive subjects having homozygote or heterozygote MBL2 variants, the salivary concentrations of IL-1β ( p = 0.010) were elevated and those of TIMP-1 ( p = 0.001) were decreased. In addition their matrix metalloproteinase -8/ TIMP-1 ratio was higher ( p < 0.001) and they had more pocket teeth ( p = 0.012) than subjects negative for A. actinomycetemcomitans. Conclusion Our findings indicate that the carriage of A. actinomycetemcomitans may facilitate extended periodontal inflammation and destruction in subjects with a variant form of human MBL2.
- Subjects
MANNOSE-binding lectins; GENETIC polymorphism research; PERIODONTITIS; GENETIC mutation; INFLAMMATION; EXONS (Genetics); SALIVA microbiology; INFECTION; GENETICS; HUMAN microbiota; GENETIC polymorphisms; INTERLEUKINS; PERIODONTAL disease; SALIVA; MATRIX metalloproteinases
- Publication
Journal of Periodontal Research, 2017, Vol 52, Issue 3, p540
- ISSN
0022-3484
- Publication type
Article
- DOI
10.1111/jre.12420