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- Title
Complete physical mapping of IL6 reveals a new marker associated with chronic periodontitis.
- Authors
Farhat, S. B.; Souza, C. M.; Braosi, A. P. R.; Kim, S. H.; Tramontina, V. A.; Papalexiou, V.; Olandoski, M.; Mira, M. T.; Luczyszyn, S. M.; Trevilatto, P. C.
- Abstract
Background and Objective Interleukin-6 ( IL-6) is a powerful stimulator of osteoclast differentiation and bone resorption. Production of IL-6 is modulated by polymorphisms, and higher levels of this cytokine are found locally in patients with chronic periodontitis. In this study we performed a modern approach - Complete physical mapping of the IL6 gene - to identify the polymorphisms associated with chronic periodontitis in a southern Brazilian population sample. Material and Methods One-hundred and nine individuals of both genders (mean age: 41.5 ± 8.5 years) were divided into a study group (56 participants with periodontitis) and a control group (53 individuals without periodontitis). After collection and purification of DNA, nine tag single nucleotide polymorphisms ( SNPs; rs1524107, rs2069835, rs2069837, rs2069838, rs2069840, rs2069842, rs2069843, rs2069845 and rs2069849) covering the entire gene were selected according to the information available on the International HapMap Project website and evaluated using real-time PCR. Results Differences in the distribution of the following parameters were statistically significant between study and control groups: number of teeth ( p = 0.030); probing depth ( p < 0.001); clinical attachment level ( p < 0.001); gingival index ( p < 0.001); plaque index ( p = 0.003); calculus index ( p < 0.001); and dental mobility ( p < 0.001). It was found that marker rs2069837 (located in intron 2 of IL6) under G dominant was associated with protection against chronic periodontitis in a Brazilian population in the presence of clinical variables, such as visible plaque, dentist visit frequency and dental floss use, and was suggested for the first time as a marker of susceptibility to chronic periodontitis. Conclusion Complete physical mapping of IL6 (using tag SNPs) was carried out for the first time, unveiling allele G of polymorphism rs2069837 (located in the second intron of IL6) as a suggestive marker of protection against chronic periodontitis in a Brazilian population.
- Subjects
GENE mapping; PERIODONTITIS; BIOMARKERS; GENOTYPES; CYTOKINES; POLYMERASE chain reaction; REVERSE transcriptase polymerase chain reaction; CONTROL groups
- Publication
Journal of Periodontal Research, 2017, Vol 52, Issue 2, p255
- ISSN
0022-3484
- Publication type
Article
- DOI
10.1111/jre.12389