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- Title
Analysis of the salivary proteome in gingivitis patients.
- Authors
Gonçalves, L. da R.; Soares, M. R.; Nogueira, F. C. S.; Garcia, C. H. S.; Camisasca, D. R.; Domont, G.; Feitosa, A. C. R.; Pereira, D. A.; Zingali, R. B.; Alves, G.
- Abstract
Gonçalves L da R, Soares MR, Nogueira FCS, Garcia CHS, Camisasca DR, Domont G, Feitosa ACR, Pereira DA, Zingali RB, Alves G. Analysis of the salivary proteome in gingivitis patients. J Periodont Res 2011; 46: 599-606.© 2011 John Wiley & Sons A/S Background and Objective: Gingivitis is a disease that is characterized by inflammation of the gingival tissue, which can progress to periodontitis and tooth loss. Although many studies have attempted to identify salivary proteins that are associated with the disease, this is the first study to use a proteomic approach to analyze and compare the proteomic profile of whole saliva from gingivitis patients and healthy controls. Material and Method: To analyze the saliva proteome, two-dimensional gel electrophoresis and liquid chromatography were used, followed by mass spectrometry. Results: The analyses showed that gingival inflammation was associated with increased amounts of blood proteins (serum albumin and hemoglobin), immunoglobulin peptides and keratins. In the control group, salivary cystatins, which were detected using capillary Liquid Chromatography on line to electrospray ionization Quadrupole Time-of-flight mass spectrometry, appeared to be more abundant. Conclusion: This approach provides novel insight into profiles of the salivary proteome during gingival inflammation, which may contribute to improvements in diagnosis.
- Subjects
BRAZIL; PROTEOMICS; BIOFILMS; METHODS in Electrophoresis; GINGIVITIS; INFLAMMATION; LIQUID chromatography; MASS spectrometry; RESEARCH funding; T-test (Statistics); EQUIPMENT &; supplies; CASE-control method; DATA analysis software; PHYSIOLOGY
- Publication
Journal of Periodontal Research, 2011, Vol 46, Issue 5, p599
- ISSN
0022-3484
- Publication type
Article
- DOI
10.1111/j.1600-0765.2011.01378.x