We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Effects of N-acyl homoserine lactone analogues on Porphyromonas gingivalis biofilm formation.
- Authors
Asahi, Y.; Noiri, Y.; Igarashi, J.; Asai, H.; Suga, H.; Ebisu, S.
- Abstract
Asahi Y, Noiri Y, Igarashi J, Asai H, Suga H, Ebisu S. Effects of N -acyl homoserine lactone analogues on Porphyromonas gingivalis biofilm formation. J Periodont Res 2009; doi: 10.1111/j.1600-0765.2009.01228.x © 2009 John Wiley & Sons A/S Background and Objective: The gram-negative anaerobic rod Porphyromonas gingivalis in oral biofilms is a primary etiological agent of periodontal disease. Biofilm formation of various gram-negative bacteria is regulated by a quorum-sensing circuit that relies on N-acyl homoserine lactones (HSLs). Some synthetic N-acyl HSL analogues act as quorum-sensing inhibitors and suppress biofilm formation in Pseudomonas aeruginosa. Development of chemical control agents against oral biofilms is necessary, because until now, biofilms have been removed only by mechanical debridement. The present study investigated the effect of N-acyl HSL analogues on P. gingivalis biofilm formation, with the aim of developing new drugs that inhibit oral biofilm formation. Material and Methods: A flow-cell model was used for P. gingivalis biofilm formation. Seventeen synthetic N-acyl HSL analogues were quantitatively assessed by spectrophotometry. The effects of three antagonistic compounds against P. gingivalis biofilm formation were further examined by confocal laser scanning microscopy, and investigated for primary attachment using spectrophotometry and phase contrast microscopy. Results: Ten out of 17 analogues affected P. gingivalis biofilm formation. Three out of 10 analogues significantly decreased biofilm-forming cells ( p < 0.05), and these biofilm structures were less well formed three-dimensionally. There were no quantitative or qualitative differences in cell attachment between the control and the three analogue-treated groups. Conclusion: Three synthetic N-acyl HSL analogues inhibited biofilm formation in P. gingivalis. We suggest that these analogues influence the development stage of P. gingivalis biofilm formation.
- Subjects
LACTONES; BIOFILMS; PORPHYROMONAS gingivalis; PSEUDOMONAS aeruginosa; DEBRIDEMENT
- Publication
Journal of Periodontal Research, 2010, Vol 45, Issue 2, p255
- ISSN
0022-3484
- Publication type
Article
- DOI
10.1111/j.1600-0765.2009.01228.x