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- Title
Role of L-Arginine on the Expression of Coagulation Factor VIII Gene.
- Authors
Ze-Wen Zhang; Da-Ming Xu; Wen-Jun Yu; Jing-Xing Yi; Jin-Feng Qiu; Cheng-Wei Xu; Chun-Ling He; Wei Fang; Xian-Ru Xu; Jie-Song Xu; Jun Yin
- Abstract
Objective • To explore the key sites in which L-arginine affects the expression of human coagulation factor VIII gene, and to create new drug targets for the treatment of hemophilia. Methods • A total of 5 human FVIII genes (A1, A2, A3, C1 and C2) with B domain deletion were transfected into human umbilical vein endothelial cells (HUVECs) as promoters. Run-on assay and ELISA analysis were performed to observe the driving effect of each domain gene on chloramphenicol acetyl transferase (CAT) gene transcription and expression, and the effect of L-arginine on each promoter. Results • In co-culture with L-arginine, transcriptional expression of the CAT gene was not detected in the PCAT3-Basic group (negative control without promoters), PA3-CAT3-Enhancer group or PC1-CAT3-Enhancer group. The transcriptional expression of CAT gene in the PCAT3-Control group (positive control with promoters) and PA1-CAT3-Enhancer group was unchanged compared with the non-L-arginine intervention, while the transcriptional expression of CAT gene in the PA2-CAT3- Enhancer group was significantly enhanced. Conclusions • A1 and A2 domain genes had promoter function and could initiate the transcription and expression of CAT gene, but A3, C1 and C2 domain genes could not. Moreover, L-arginine can significantly enhance transcription and expression of human coagulation factor VIII via A2 domain.
- Subjects
ARGININE; UMBILICAL veins; ENDOTHELIAL cells; ENZYME-linked immunosorbent assay; GENETIC transcription
- Publication
Alternative Therapies in Health & Medicine, 2023, Vol 29, Issue 3, p26
- ISSN
1078-6791
- Publication type
Article