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- Title
Ajugol's upregulation of TFEB-mediated autophagy alleviates endoplasmic reticulum stress in chondrocytes and retards osteoarthritis progression in a mouse model.
- Authors
Wu, Jingtao; Yu, Heng; Jin, Yangcan; Wang, Jingquan; Zhou, Liwen; Cheng, Teng; Zhang, Zhao; Lin, Binghao; Miao, Jiansen; Lin, Zhongke
- Abstract
Background: Osteoarthritis (OA), a degenerative disease with a high global prevalence, is characterized by the degradation of the extracellular matrix (ECM) and the apoptosis of chondrocytes. Ajugol, a extract derived from the herb Rehmannia glutinosa, has not yet been investigated for its potential in modulating the development of OA. Methods: We employed techniques such as western blotting, immunofluorescence, immunohistochemistry, X-ray imaging, HE staining, and SO staining to provide biological evidence supporting the role of Ajugol as a potential therapeutic agent for modulating OA. Furthermore, in an in vivo experiment, intra-peritoneal injection of 50 mg/kg Ajugol effectively mitigated the progression of OA following destabilization of the medial meniscus (DMM) surgery. Results: Our findings revealed that treatment with 50 μM Ajugol activated TFEB-mediated autophagy, alleviating ER stress-induced chondrocyte apoptosis and ECM degradation caused by TBHP. Furthermore, in an in vivo experiment, intra-peritoneal injection of 50 mg/kg Ajugol effectively mitigated the progression of OA following destabilization of the medial meniscus (DMM) surgery. Conclusion: These results provide compelling biological evidence supporting the role of Ajugol as a potential therapeutic agent for modulating OA by activating autophagy and attenuating ER stress-induced cell death and ECM degradation. The promising in vivo results further suggest the potential of Ajugol as a treatment strategy for OA progression.
- Subjects
PHYTOTHERAPY; CARTILAGE cells; DISEASE progression; BIOLOGICAL models; DRUG efficacy; HERBAL medicine; STAINS &; staining (Microscopy); TERPENES; IN vivo studies; INJECTIONS; ENDOPLASMIC reticulum; AUTOPHAGY; ANIMAL experimentation; WESTERN immunoblotting; IMMUNOHISTOCHEMISTRY; GLYCOSIDES; MENISCECTOMY; APOPTOSIS; OSTEOARTHRITIS; FLUORESCENT antibody technique; RESEARCH funding; PLANT extracts; MOLECULAR structure; EXTRACELLULAR space; MICE; EVALUATION
- Publication
Chinese Medicine, 2023, Vol 18, Issue 1, p1
- ISSN
1749-8546
- Publication type
Article
- DOI
10.1186/s13020-023-00824-7