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- Title
Immobilization of β-glucosidase onto Magnetic Nanoparticles and Evaluation of the Enzymatic Properties.
- Authors
Ying Zhou; Siyi Pan; Xuetuan Wei; Lufeng Wang; Yanlong Liu
- Abstract
This paper reports on a novel and efficient β-glucosidase immobilization method using magnetic Fe3O4 nanoparticles as a carrier. Based on response surface methodology, the optimal immobilization conditions obtained were: glutaraldehyde (GA) concentration, 0.20%; enzyme concentration, 50.25 μg/mL; cross-linking time, 2.21 h; and the maximum activity recovery reached 89.35%. The magnetic immobilized enzyme was characterized by Fourier transform infrared spectroscopy (FTIR), transmission electron microscope (TEM), and vibrating sample magnetometer (VSM). FTIR revealed that β-glucosidase was successfully immobilized on the magnetic nanoparticles. TEM showed that enzyme-magnetic nanoparticles possessed nano-scale size distribution. VSM confirmed that the enzyme-magnetic nanoparticles were superparamagnetic. The properties of the immobilized β-glucosidase were improved, and the immobilized β-glucosidase exhibited wider pH and temperature ranges of activation, higher accessibility of the substrate, better thermal stability, and better storage stability than that of the free enzyme. The enzyme-magnetic nanoparticles could be separated magnetically for easy reuse. Immobilization of β-glucosidase onto the magnetic nanoparticles has the potential for industrial application.
- Subjects
BETA-glucosidase; IRON oxide nanoparticles; RESPONSE surfaces (Statistics); GLUTARALDEHYDE; CROSSLINKING (Polymerization); IMMOBILIZED enzymes; FOURIER transform infrared spectroscopy; TRANSMISSION electron microscopes
- Publication
BioResources, 2013, Vol 8, Issue 2, p2605
- ISSN
1930-2126
- Publication type
Article
- DOI
10.15376/biores.8.2.2605-2619