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- Title
Attempt at cloning high-quality goldfish breed ‘Ranchu’ by fin-cultured cell nuclear transplantation.
- Authors
Tanaka, Daisuke; Takahashi, Akito; Takai, Akinori; Ohta, Hiromi; Ueno, Koichi
- Abstract
The viability of ornamental fish culture relies on the maintenance of high-quality breeds. To improve the profitability of culture operations we attempted to produce cloned fish from the somatic nucleus of the high-quality Japanese goldfish (Carassius auratus auratus) breed ‘Ranchu’. We transplanted the nucleus of a cultured fin-cell from an adult Ranchu into the non-enucleated egg of the original goldfish breed ‘Wakin’. Of the 2323 eggs we treated, 802 underwent cleavage, 321 reached the blastula stage, and 51 reached the gastrula stage. Two of the gastrulas developed until the hatching stage. A considerable number of nuclear transplants retained only the donor nucleus. Some of these had only a 2n nucleus derived from the same donor fish. Our results provide insights into the process of somatic cell nuclear transplantation in teleosts, and the cloning of Ranchu.
- Subjects
GOLDFISH; MOLECULAR cloning; FISH quality; ORNAMENTAL fishes; BLASTULA; FISH development; OSTEICHTHYES
- Publication
Zygote, 2012, Vol 20, Issue 1, p79
- ISSN
0967-1994
- Publication type
Article
- DOI
10.1017/S0967199410000560