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- Title
Automatic microfluidic fluorescence-array measurement system for detecting organic phosphate.
- Authors
Hsing-Cheng Chang; Jung-Chin Lin; Shyan-Lung Lin; I-Nan Chang; Chern-Sheng Lin; Shi-Yao Chen; Chang, Hsing-Cheng; Lin, Jung-Chin; Lin, Shyan-Lung; Chang, I-Nan; Lin, Chern-Sheng; Chen, Shi-Yao
- Abstract
In this study, an automatic microfluidic fluorescence-array measurement system is developed to detect the concentration of organic phosphate based on the luminol-hydrogen peroxide catalytic fluorescent mechanism. Not only sample quantity and cost can be reduced, but also detection time, accuracy and precision can be improved in the system. The system is composed of a CCD image module, a stepper motor with driver, a microfluidic fluorescence array, a background light elimination module, and a dynamic image-analyzed interface. The pesticides of chlorpyrifos and fenitrothion of organic phosphate are chosen as experimental samples. Only a 2.5 μ l quantity of sample is required to have a fast response time of 1.4 second. Experimental results show that the sensitivities of chlorpyrifos and fenitrothion are 1.88 V/ppm in the range of 0.166 ∼ 10 ppm with averaged error of 1.66% and 0.32 V/ppm in the range of 0.03 ∼ 10 ppm with averaged error of 1.68% respectively. The organophosphorus effective detection range of the developed system covers the legal prescription for pesticide residues.
- Subjects
FLUORESCENCE; PHOSPHATES; CHLORPYRIFOS; FENITROTHION; ORGANOPHOSPHORUS pesticides; PESTICIDE analysis; LIGHT; ORGANOPHOSPHORUS compounds; ORGANOTHIOPHOSPHORUS compounds; MICROFLUIDIC analytical techniques
- Publication
Technology & Health Care, 2016, Vol 24, pS41
- ISSN
0928-7329
- Publication type
journal article
- DOI
10.3233/THC-151050