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- Title
Expression of the atf1<sup>+</sup> gene is upregulated in fission yeast under nitrosative and nutritional stresses.
- Authors
Song, S.-H.; Kim, B.-M.; Lim, C.-J.; Song, Y. S.; Park, E.-H.
- Abstract
This work was designed to assess regulation of the atf1+ gene in the fission yeast Schizosaccharomyces pombe under nitrosative and nutritional stresses, using the atf1+-lacZ fusion gene and RT-PCR. Nitric oxide (NO)-generating sodium nitroprusside (SNP; 10 µmol/L) and nitrogen depletion significantly enhanced synthesis of β-galactosidase from the atf1+-lacZ fusion gene in S. pombe Pap1-positive KP1 cells, but not in S. pombe Pap1-negative TP108-3C cells. SNP (10 µmol/L) and nitrogen depletion also caused a significant increase in atf1+ mRNA levels in Pap1-positive cells, but not in Pap1-negative cells. Depletion of glucose marginally increased synthesis of β-galactosidase from the fusion gene in S. pombe Pap1-positive cells. Taken together, the S. pombe atf1+ gene is upregulated by nitrosative and nutritional stresses on a transcriptional level, possibly via the mediation of Pap1.
- Subjects
YEAST; SCHIZOSACCHAROMYCES pombe; SCHIZOSACCHAROMYCES; GENES; SODIUM nitroferricyanide; MESSENGER RNA
- Publication
Canadian Journal of Microbiology, 2009, Vol 55, Issue 11, p1323
- ISSN
0008-4166
- Publication type
Article
- DOI
10.1139/W09-087