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- Title
AN ANTI-LIPOPOLYSACCHARIDE FACTOR IN LITOPENAEUS VANNAMEI PARTICIPATES IN THE IMMUNE DEFENSE AGAINSTWSSV AND VIBRIO ANGUILLARUM.
- Authors
Wenbin Zhan; Liangyin He; Xiumei Wei; Xiaolu Wang; Xiaoqian Tang
- Abstract
The anti-lipopolysaccharide factors (ALFs) are a group of effect or molecules of innate immunity in arthropods, exhibiting binding and neutralizing activities to lipopolysaccharides. In the present paper, an ALF gene from shrimp Litopenaeus vannamei (Boone, 1931) (LvALF) was cloned by rapid amplification of cDNA ends (RACE) technique, which encodes a polypeptide of 122 amino acids with a predicted molecular mass of 13.7 kDa and a theoretical isoelectric point of 9.95. In healthy shrimp, the LvALF mRNA was highly expressed in hemocytes, and moderately in gill and heart, whereas low in muscle, intestine and hepatopancres. While the healthy shrimps were challenged with white spot syndrome virus (WSSV) and Vibrio anguillarum, the LvALF mRNA level in hemocytes was significantly up-regulated and reached peak levels at 6-12 h and then gradually decreased to the control level. Moreover, the LPS-binding activity of recombinant LvALF (rLvALF) was confirmed by ELISA and modified pull-down assay using the polyclonal antibodies against the rLvALF, meanwhile the up-regulated expressions of LvALF in hemocytes after challenges with WSSV or V. anguillarum were also detected at protein and cellular levels by Western blotting and immunocytochemistry. These results indicated that LvALF was involved in the immune defense against WSSV and V. anguillarum.
- Subjects
WHITELEG shrimp; PENAEUS; ARTHROPODA; NATURAL immunity; LIPOPOLYSACCHARIDES; SHRIMP diseases; WHITE spot syndrome virus; PHYSIOLOGY
- Publication
Journal of Crustacean Biology, 2015, Vol 35, Issue 5, p670
- ISSN
0278-0372
- Publication type
Article
- DOI
10.1163/1937240X-00002364