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- Title
MOLECULAR EPIDEMIOLOGICAL STUDY ON PESTE DES PETITS RUMINANTS IN EGYPT 2015.
- Authors
ElAshmawy, Wagdy R.; Bazid, Abdelhamid I.; Aboelkhair, Mohamed; Sakr, Mostafa A.; Fayed, Aysam M.; Fawzy, Mohamed
- Abstract
Peste des petits ruminants (PPR) is one of highly contagious viral diseases of small ruminants with high economic losses due to the high morbidity and mortality. In Egypt, PPR in the last 10 years re-emerged again with high mortality in sheep flocks. There is no much data regarding the circulating Peste des petits ruminants' virus and the epidemiological distribution in small ruminants. The study was carried out on a sheep flock of 50 rams aged of 9-12 months with clinical signs suggestive to PPR infection (fever, erosions of the buccal mucosa, pneumonia, diarrhea high morbidity and mortality). Buffy coat and lymph nodes from diseased and dead animals were sent for diagnosis and molecular diagnosis was confirmed using RT-PCR with specific primers targeting three genes; nucleo-capsid (N), fusion (F), and hemagglutinin (H). Sequencing and phylogenetic analysis was carried out on the PCR products and revealed that, the circulating virus is belonged to lineage IV along with Ethiopian strain.
- Subjects
MOLECULAR epidemiology; PESTE des petits ruminants; BUFFY coat; HEMAGGLUTININ; PNEUMONIA
- Publication
Slovenian Veterinary Research / Slovenski Veterinarski Zbornik, 2018, Vol 55, Issue 4, p205
- ISSN
1580-4003
- Publication type
Article
- DOI
10.26873/SVR-237-2018