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- Title
E-Cadherin Regulates Neural Stem Cell Self-Renewal.
- Authors
Karpowicz, Phillip; Willaime-Morawek, Sandrine; Balenci, Laurent; DeVeale, Brian; Inoue, Tomoyuki; van der Kooy, Derek
- Abstract
E-Cadherin, a cell adhesion protein, has been shown to take part in the compartmentalization, proliferation, survival, and differentiation of cells. E-Cadherin is expressed in the adult and embryonic forebrain germinal zones in vivo, and in clonal colonies of cells derived from these regions and grown in vitro. Mice carrying E-Cadherin floxed genes crossed to mice expressing Cre under the Nestin promoter demonstrate defects in the self-renewal of neural stem cells both in vivo and in vitro. The functional role of E-Cadherin is further demonstrated using adhesion-blocking antibodies in vitro, which specifically target cadherin extracellular adhesive domains. Adult neural stem cell colonies decrease in the presence of E-Cadherin antibodies in a dosage-dependent manner, in contrast to P-Cadherin antibody. On overexpression of normal E-Cadherin and a mutated E-Cadherin, containing no intracellular binding domain, an increased number of clonal adult neural stem cell colonies are observed. These data suggest it is specifically E-Cadherin adhesion that is responsible for these self-renewal effects. These data show the importance of E-Cadherin in the neural stem cell niche and suggest E-Cadherin regulates the number of these cells.
- Subjects
CADHERINS; CELL adhesion molecules; CELL proliferation; CELL differentiation; NEURAL stem cells; IMMUNOGLOBULINS
- Publication
Journal of Neuroscience, 2009, Vol 29, Issue 12, p3885
- ISSN
0270-6474
- Publication type
Article
- DOI
10.1523/JNEUROSCI.0037-09.2009