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- Title
CCAAT/enhancer binding protein and nuclear factor-Y regulate adiponectin gene expression in adipose tissue.
- Authors
Park, Sang-kyu; Oh, So-Young; Lee, Min-Young; Yoon, Sarah; Kim, Kyung-Sup; Kim, Jae-woo
- Abstract
Adiponectin is one of the adipokines secreted by adipocytes and regulates energy homeostasis associated with insulin sensitivity, suggesting a possibility of nutritional regulation of adiponectin gene expression. In this study, we showed that the transcription of adiponectin gene was induced 4-6 h after refeeding of mice. Also, differentiated 3T3-L1 adipocytes that were treated with high glucose expressed significantly increased adiponectin mRNA. Promoter analysis using nuclear extracts from white adipose tissue revealed that CCAAT/enhancer binding protein (C/EBP) and nuclear factor-Y (NF-Y) bound on the -117/-73 region of the adiponectin promoter. This region was critical for the activity of the adiponectin promoter as the deletion or mutation of this region markedly diminished the promoter activity to a basal level. Furthermore, the C/EBP binding increased in both refed animal and high glucose-treated 3T3-L1 adipocytes in an electrophoretic mobility shift assay, suggesting that C/EBP is responsible for the dietary response of the adiponectin gene expression. Chromatin immunoprecipitation studies demonstrated the binding of C/EBP and NF-Y in both mouse and differentiated 3T3-L1 adipocytes and also that C/EBP binding increased in response to high glucose. These findings demonstrated that C/EBP and NF-Y are critical for the regulation of the adiponectin expression in response to nutrients and in the course of adipocyte differentiation.
- Subjects
FAT cells; HOMEOSTASIS; INSULIN; NUCLEOPROTEINS; ADIPOSE tissues; PROTEIN metabolism; ANIMAL experimentation; BINDING sites; CELL differentiation; CELL nuclei; COMPARATIVE studies; DOCUMENTATION; ESTERASES; GENES; GENETIC techniques; GROWTH factors; RESEARCH methodology; MEDICAL cooperation; MICE; NUCLEIC acid hybridization; NUCLEOTIDES; POLYMERASE chain reaction; RESEARCH; RNA; TRANSCRIPTION factors; EVALUATION research; REVERSE transcriptase polymerase chain reaction; ADIPONECTIN; PHYSIOLOGY
- Publication
Diabetes, 2004, Vol 53, Issue 11, p2757
- ISSN
0012-1797
- Publication type
journal article
- DOI
10.2337/diabetes.53.11.2757