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- Title
Anti-Cancer Properties of Diethylether Extract of Wood from Sukun (Artocarpus altilis) in Human Breast Cancer (T47D) Cells.
- Authors
Arung, Enos Tangke; Wicaksono, Britanto Dani; Handoko, Yohana Ayupriyanti; Kusuma, Irawan Wijaya; Yulia, Dina; Sandra, Ferry
- Abstract
Purpose: To evaluate the anti-cancer properties of the diethylether extract of Sukun (Artocarpus altilis) wood. Methods: The extract was tested in human T47D breast cancer cells and examined for its effect on cell viability, nuclear morphology and sub-G1 formation. Cell viability was determined by microculture tetrazolium technique (MTT), nuclear morphology investigated using 4'-6-diamidino-2-phenylindole (DAPI) staining technique, and cell cycle progression monitored by sub-G1 apoptosis assay using flow cytometry. Results: The results showed decreasing cell viability in a concentration-dependent manner. Altered cell morphology after treatment with the extract demonstrated that cells experienced apoptosis. Cell cycle analysis indicated that the number of cells in sub-G1 phase rose with increasing concentrations of the extract. Conclusion: The data demonstrate that Sukun wood extract induced apoptosis and sub-G1 phase formation in breast cancer (T47D) cells, and therefore, has a potential as an anti-cancer agent.
- Subjects
BREAST cancer treatment; CANCER treatment; ARTOCARPUS; CANCER cells; CELL morphology; TETRAZOLIUM; STAINS &; staining (Microscopy); CELL cycle; APOPTOSIS; CYTOMETRY; PHYSIOLOGY
- Publication
Tropical Journal of Pharmaceutical Research, 2009, Vol 8, Issue 4, p317
- ISSN
1596-5996
- Publication type
Article
- DOI
10.4314/tjpr.v8i4.45223