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- Title
Gastroprotective mechanism of Paeder foetida Linn. (Rubiaceae) -- a popular edible plant used by the tribal community of North-East India.
- Authors
Chanda, Silpi; Deb, Lokesh; Tiwari, Raj Kumar; Singh, Kuldeep; Ahmad, Sayeed
- Abstract
Background: The objective of the present study is to evaluate the gastroprotective activity of Paederia foetida L. leaf for gastric ulcer. Methods: The methanol extract of P. foetida L. leaves at two different dose levels was investigated for gastroprotective potential by using Indomethacin-pylorus ligation, alcohol induced and water immersion stress induced model in rats. In vitro DPPH* radical scavenging activity and western blot analysis of stomach tissue from pylorus ligatures rats were also carried out. HPTLC analysis was done to understand the phytopharmacological relationship. Results: Methanol extract at a dose level of 100 mg/kg and 200 mg/kg body weight showed 72 and 78 % ulcer protection when compared to negative control whereas reference drug shown 82 % protection in Indomethacin-pylorus ligation model. Further, methanol extract also showed protective effect against 70 % v/v ethanol and stress induced gastric ulcer model. About 84 % protection as compared to cimetidine (85 %) was seen in western blot analysis of stomach tissue from pylorus ligatures rats. HPTLC analysis of methanol extract of P. foetida L. confirmed the presence of β-sitosterol. In DPPH* radical scavenging activity, the IC50 value was observed to be 43.52 ng/ml. Conclusions: These observations established the traditional claim and thus Paederia foetida could be a potent gastroprotective agent for use in future. The gastroprotective activity might be mediated by the Nrf2 mediated antioxidant and anti secretory effects.
- Subjects
INDIA; ULCER prevention; ANIMAL experimentation; ANTIOXIDANTS; GASTROINTESTINAL system; HIGH performance liquid chromatography; LEAVES; METHANOL; RATS; WESTERN immunoblotting; PLANT extracts; DATA analysis software; DESCRIPTIVE statistics; ONE-way analysis of variance
- Publication
BMC Complementary & Alternative Medicine, 2015, Vol 15, Issue 1, p1
- ISSN
1472-6882
- Publication type
Article
- DOI
10.1186/s12906-015-0831-0