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- Title
study of interferon-γ, transforming growth factor-β, and interleukin-4 gene expression induced by radioadaptive response.
- Authors
Bahreyni-Toossi, Mohammad-Taghi; Sankian, Mojtaba; Azimian, Hosein; Najafi-Amiri, Maryam; Khademi, Sara; Abdollahi-Dehkordi, Sepideh; Hamzian, Nima
- Abstract
<bold>Introduction: </bold>In the present study, the radioadaptive role of the immune system induced by low dose (LD) was investigated for its in vivo protective activity.<bold>Materials and Methods: </bold>Quantitative analysis of cytokine gene expression was assessed for their in vivo activity in BALB/c mice. To evaluate the adaptive response induced by LD on the mice spleen lymphocyte, the cytokine interleukin (IL)-4, interferon (IFN)-γ, and transforming growth factor (TGF)-β expression was measured by a real-time quantitative polymerase chain reaction. To verify the radioadaptive effect of LD, animals were preirradiated at 10 cGy from a 60 Co source and then challenge dose at 200 cGy was delivered. Independent sample student's t-test was employed to compare cytokine gene expression in radioadaptive (10 + 200 cGy), LD (10 cGy), high-dose (HD, 200 cGy), and control groups of animals.<bold>Results: </bold>Following the HD, the cytokine gene expression of IFN-γ, IL-4, and TGF-β was significantly decreased compared to the control group (P = 0.0001). However, TGF-β expression was also decreased significantly in the LD and adaptive groups compared to the control group (P = 0.0001). IFN-γ/IL-4 ratio in the adaptive group was significantly decreased compared to the HD group (P = 0.0001).<bold>Conclusion: </bold>These results indicate that the immune system plays an important role for radioadaptive response induction by LD radiation to adjust the harmful effects of HD irradiation.
- Subjects
GENE expression; TRANSFORMING growth factors; INTERLEUKIN-4; CYTOKINES; IN vivo studies; INTERLEUKINS; BIOLOGICAL models; CELL culture; GROWTH factors; ANIMAL experimentation; PHYSIOLOGICAL adaptation; INTERFERONS; DOSE-response relationship (Radiation); IMMUNITY; GENES; RADIOTHERAPY; T cells; MICE; PHYSIOLOGICAL effects of radiation
- Publication
Journal of Cancer Research & Therapeutics, 2021, Vol 17, Issue 2, p537
- ISSN
0973-1482
- Publication type
journal article
- DOI
10.4103/jcrt.JCRT_71_19