We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Senile hair graying: H<sub>2</sub>O<sub>2</sub>-mediated oxidative stress affects human hair color by blunting methionine sulfoxide repair.
- Authors
Wood, J. M.; Decker, H.; Hartmann, H.; Chavan, B.; Rokos, H.; Spencer, J. D.; Hasse, S.; Thornton, M. J.; Shalbaf, M.; Paus, R.; Schallreuter, K. U.
- Abstract
Senile graying of human hair has been the subject of intense research since ancient times. Reactive oxygen species have been implicated in hair follicle melanocyte apoptosis and DNA damage. Here we show for the first time by FT-Raman spectroscopy vivo that human gray/white scalp hair shafts accumulate hydrogen peroxide (H2O2) in millimolar concentrations. Moreover, we demonstrate almost absent catalase and methionine sulfoxide reductase A and protein expression via immunofluorescence and Western blot in association with a functional loss of methionine sulfoxide (Met-S=O) repair in the entire gray hair follicle. Accordingly, Met-S=O formation of Met residues, including Met 374 in the active site of tyrosinase, the key enzyme in melanogenesis, limits enzyme functionality, as evidenced by FT-Raman spectroscopy, computer simulation, and enzyme kinetics, which leads to gradual loss of hair color. Notably, under in vitro conditions, Met oxidation can be prevented by L-methionine. In summary, our data feed the long-voiced, but insufficiently proven, concept of H2O2-induced oxidative damage in the entire human hair follicle, inclusive of the hair shaft, as a key element in senile hair graying, which does not exclusively affect follicle melanocytes. This new insight could open new strategies for intervention and reversal of the hair graying process.
- Subjects
GRAY hair; OXIDATIVE stress; HYDROGEN peroxide; METHIONINE; HAIR follicles; RAMAN spectroscopy
- Publication
FASEB Journal, 2009, Vol 23, Issue 7, p2065
- ISSN
0892-6638
- Publication type
Article
- DOI
10.1096/fj.08-125435