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- Title
Nuclear Microsatellite Primers for the Endangered Relict Fir, Abies pinsapo (Pinaceae) and Cross-Amplification in Related Mediterranean Species.
- Authors
Sánchez-Robles, Jose M.; Balao, Francisco; García-Castaño, Juan L.; Terrab, Anass; Navarro-Sampedro, Laura; Talavera, Salvador
- Abstract
Twelve nuclear microsatellite primers (nSSR) were developed for the endangered species Abies pinsapo Boiss to enable the study of gene flow and genetic structure in the remaining distribution areas. Microsatellite primers were developed using next-generation sequencing (454) data from a single Abies pinsapo individual. Primers were applied to thirty individuals from the three extant localities. The number of alleles per locus ranged from one to four. Cross-amplification was tested for other Abies species from the Mediterranean Basin, and most of the loci showed higher polymorphisms in the Mediterranean species than in A. pinsapo. These microsatellite markers provide tools for conservation genetic studies in Abies pinsapo as well other Abies species from the Mediterranean Basin.
- Subjects
MEDITERRANEAN Region; SPANISH fir; ENDANGERED species; MICROSATELLITE repeats; GENE amplification; GENETIC polymorphisms; PLANT genetics
- Publication
International Journal of Molecular Sciences, 2012, Vol 13, Issue 11, p14243
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms131114243