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- Title
Single-cell microfluidics in combination with chlorophyll a fluorescence measurements to assess the lifetime of the Chlamydomonas PSBO protein.
- Authors
SZÉLES, E.; KUNTAM, S.; VIDAL-MEIRELES, A.; NAGY, V.; NAGY, K.; ÁBRAHÁM, Á.; KOVÁCS, L.; TÓTH, S. Z.
- Abstract
PSBO is an essential subunit of the oxygen-evolving complex and we recently demonstrated that its lifetime depends on environmental conditions in Chlamydomonas reinhardtii. To assess PSBO lifetime with a high time resolution, we employed (1) a microfluidic platform enabling the trapping of single cells and the parallel measurement of photosynthetic activity, and (2) a nitrate-inducible PSBO amiRNA line. Our microfluidic platform allowed the rapid replacement of the nutrient solution necessary for induction. It also enabled the precise monitoring of the decline in the Fv/Fm value, reflecting PSBO loss. We found that in the dark, at medium and high light intensity, the Fv/Fm value decreased with halftimes of about 25, 12.5, and 5 h, respectively. We also observed that photosynthetic activity was better sustained upon carbon limitation. In the absence of acetate, the halftimes of Fv/Fm diminishment doubled to quadrupled compared with the control, acetate-supplied cultures.
- Subjects
CHLOROPHYLL spectra; CHLAMYDOMONAS; MICROFLUIDICS; CHLAMYDOMONAS reinhardtii; LIGHT intensity
- Publication
Photosynthetica, 2023, Vol 61, p417
- ISSN
0300-3604
- Publication type
Article
- DOI
10.32615/ps.2023.028