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- Title
Microdomains heterogeneity in the thylakoid membrane proteins visualized by super-resolution microscopy.
- Authors
KAŇA, R.; ŠEDIVÁ, B.; PRÁŠIL, O.
- Abstract
The investigation of spatial heterogeneity within the thylakoid membrane (TM) proteins has gained increasing attention in photosynthetic research. The recent advances in live-cell imaging have allowed the identification of heterogeneous organisation of photosystems in small cyanobacterial cells. These sub-micrometre TM regions, termed microdomains in cyanobacteria, exhibit functional similarities with granal (Photosystem II dominant) and stromal (Photosystem I dominant) regions observed in TM of higher plants. This study delves into microdomain heterogeneity using super- resolution Airyscan-based microscopy enhancing resolution to approximately ~125 nm in x-y dimension. The new data reveal membrane areas rich in Photosystem I within the inner TM rings. Moreover, we identified analogous dynamics in the mobility of Photosystem II and phycobilisomes; countering earlier models that postulated differing mobility of these complexes. These novel findings thus hold significance for our understanding of photosynthesis regulation, particularly during state transitions.
- Subjects
MEMBRANE proteins; PHOTOSYSTEMS; HETEROGENEITY; MICROSCOPY; PHYCOBILISOMES
- Publication
Photosynthetica, 2023, Vol 61, p483
- ISSN
0300-3604
- Publication type
Article
- DOI
10.32615/ps.2023.043