We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
A multiplex RT-PCR method to detect papaya meleira virus complex in adult pre-flowering plants.
- Authors
Maurastoni, Marlonni; Sá-Antunes, Tathiana F.; Oliveira, Scarlett A.; Santos, Alexandre M. C.; Ventura, José A.; Fernandes, Patricia M. B.
- Abstract
Papaya sticky disease (PSD), which can destroy orchards, was first attributed to papaya meleira virus (PMeV). However, the discovery of papaya meleira virus 2 (PMeV2) associated with PSD plants impose the need to detect this viral complex. We developed a multiplex RT-PCR (mPCR) technique capable of detecting two viruses in a single assay from pre-flowering plant samples, which is a useful tool for early diagnosis of PSD. We also determined the limit of detection (LOD) using asymmetric plasmid dilutions of both PMeV and PMeV2, which revealed that a higher titer of one virus prevents detection of the other. Thus, this technique is an alternative method for detecting PMeV and PMeV2 in a single reaction.
- Subjects
REVERSE transcriptase polymerase chain reaction; PAPAYA; VIRUSES; DETECTION limit; PLANTS; EARLY diagnosis
- Publication
Archives of Virology, 2020, Vol 165, Issue 5, p1211
- ISSN
0304-8608
- Publication type
Article
- DOI
10.1007/s00705-020-04588-5