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- Title
Identification of a novel envelope protein encoded by ORF 136 from Cyprinid herpesvirus 3.
- Authors
Zheng, Shucheng; Li, Yingying; Wang, Qing; Wu, Jiexing; Wang, Yingying; Zeng, Weiwei; Bergmann, Sven; Ren, Yan; Shi, Cunbin
- Abstract
Cyprinid herpesvirus 3 (CyHV-3) is the pathogenic agent of koi herpesvirus disease (KHVD) afflicting common carp and koi ( Cyprinus carpio L.) populations globally. As described previously, proteomic analyses of purified CyHV-3 particles have shown that at least 46 structural proteins are incorporated into CyHV-3 virions; among these ORF136 may encode a putative envelope protein. In this study, Western blotting analysis showed that a specific band with the predicted molecular weight of 17 kDa was detected both in purified virions and envelope components using a rabbit anti-ORF136 polyclonal antibody. Indirect immunofluorescence assay with confocal laser scanning microscopy indicated that the ORF136 protein was distributed in the cytoplasm of CCB cells infected with CyHV-3 and transfected with a pVAX1-ORF136 plasmid. Furthermore, immunogold electron microscopy confirmed that ORF136 protein localized to the CyHV-3 envelope.
- Subjects
HERPESVIRUSES; VIRAL proteins; CARP; IMMUNOLOGY; VIRUS diseases; CYPRINUS; OPEN reading frames (Genetics); DISEASES; VIRUSES
- Publication
Archives of Virology, 2017, Vol 162, Issue 12, p3849
- ISSN
0304-8608
- Publication type
Report
- DOI
10.1007/s00705-017-3528-5