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- Title
Molecular characterization of divergent grapevine Pinot gris virus isolates and their detection in Slovak and Czech grapevines.
- Authors
Glasa, Miroslav; Predajňa, Lukáš; Komínek, Petr; Nagyová, Alžbeta; Candresse, Thierry; Olmos, Antonio
- Abstract
Analysis of complete genome sequences of three Slovak isolates of grapevine Pinot gris virus (GPGV) showed their low heterogeneity (reaching 1.7 %) and a close relationship to the Italian NC_015782 isolate (4.2-4.5 % divergence). Comparison of Slovak and Italian isolates revealed an unusual accumulation of 21 indel mutations in ORF1, resulting in a localized high divergence in the encoded amino acid sequences. An elevated divergence in the 5′ extremity of the GPGV genomes is suggestive of a recombination between Slovak isolates and grapevine berry inner necrosis virus. RT-PCR allowed the frequent detection of closely related GPGV isolates in grapevines from Slovakia and the Czech Republic.
- Subjects
SLOVAKIA; CZECH Republic; MOLECULAR virology; GRAPE varieties; GRAPES; NUCLEOTIDE sequence; OPEN reading frames (Genetics); VIRUSES
- Publication
Archives of Virology, 2014, Vol 159, Issue 8, p2103
- ISSN
0304-8608
- Publication type
Report
- DOI
10.1007/s00705-014-2031-5