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- Title
Augmentation of Mitochondrial Reduced Glutathione by S-Adenosyl-L-Methionine Administration in Ischemia-Reperfusion Injury of the Rat Steatotic Liver Induced by Choline-Methionine-Deficient Diet.
- Authors
Kaneshiro, Y.; Nakano, H.; Kumada, K.; Boudjema, K.; Kitamura, N.; Shimura, H.; Barama, A.; Kigawa, G.; Tatsuno, M.; Fujiwara, Y.; Baek, Y.; Sasaki, J.; Nagasaki, H.; Yamaguchi, M.
- Abstract
We examined whether warm ischemia-reperfusion (I/R) damage of the rat steatotic liver can be reduced by administration of S-adenosyl-L-methionine (SAMe). We examined the effect of SAMe on the mitochondrial reduced-glutathione (GSH) pool. Sixty minutes of partial left lobar vascular clamping followed by 2 h of reperfusion were employed for a model of hepatic warm ischemia. Either 5% dextrose or SAMe was injected intraperitoneally 2 h before I/R in steatotic rats (S-D5% or S-SAMe group). Serum liver enzyme concentrations 2 h after reperfusion were significantly lower in the S-SAMe group than in the S-D5% group. The cytosolic and mitochondrial GSH concentrations after I/R were significantly higher in the S-SAMe group than in the S-D5% group (p < 0.05). The cytosolic and mitochondrial oxidized-glutathione/GSH ratios after I/R were significantly greater in the S-D5% group than in the S-SAMe group (p < 0.01). The adenosine triphosphate concentration was higher in the S-SAMe group than in the S-D5% group (p = 0.0515). These results show that hepatocellular and mitochondrial oxidative stress after I/R in the steatotic liver can be reduced by administration of SAMe. The results also show that mitochondrial function and hepatocellular integrity can be restored by administration of SAMe in steatotic rats.
- Subjects
MYOCARDIAL reperfusion; ADENOSYLMETHIONINE; GLUTATHIONE; CHOLINE; METHIONINE; THERAPEUTICS
- Publication
European Surgical Research, 1998, Vol 30, Issue 1, p34
- ISSN
0014-312X
- Publication type
Article
- DOI
10.1159/000008555