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- Title
A novel protein-mineral interface.
- Authors
Alexeev, Dmitriy; Zhu, Haizhong; Guo, Maolin; Zhong, Weiqing; Hunter, Dominic J.B.; Yang, Weiping; Campopiano, Dominic J.; Sadler, Peter J.
- Abstract
Transferrins transport Fe3+ and other metal ions in mononuclear-binding sites. We present the first evidence that a member of the transferrin superfamily is able to recognize multi-nuclear oxo-metal clusters, small mineral fragments that are the most abundant forms of many metals in the environment. We show that the ferric ion-binding protein from Neisseria gonorrhoeae (nFbp) readily binds clusters of Fe3+, Ti4+, Zr4+ or Hf4+ in solution. The 1.7 A resolution crystal structure of Hf-nFbp reveals three distinct types of clusters in an open, positively charged cleft between two hinged protein domains. A di-tyrosyl cluster nucleation motif (Tyr195-Tyr196) is situated at the bottom of this cleft and binds either a trinuclear oxo-Hf cluster, which is capped by phosphate, or a pentanuclear cluster, which in turn can be capped with phosphate. This first high-resolution structure of a protein-mineral interface suggests a novel metal-uptake mechanism and provides a model for protein-mediated mineralization/dissimilation, which plays a critical role in geochemical processes.NOTE: In the version of this article initially published online, the institution affiliations were assigned incorrectly because of a mistake that occurred during production. The correct affiliations for all authors are as follows: Dmitriy Alexeev1, Haizhong Zhu2, Maolin Guo2,3, Weiqing Zhong2,4, Dominic J.B. Hunter2, Weiping Yang2,3, Dominic J. Campopiano2 and Peter J. Sadler2. All of the footnotes (corrected) are as follows: 1Institute of Cell and Molecular Biology, Michael Swann Building, University of Edinburgh, Mayfield Road, Edinburgh EH9 3JR, UK; 2School of Chemistry, University of Edinburgh, West Mains Road, Edinburgh EH9 3JJ, UK; 3Current address: Department of Molecular Biology and Biochemistry, University of California, Irvine, California 92697, USA; and 4Current address: School of Pharmacy, Second Military Medicine University, Shanghai...
- Subjects
TRANSFERRIN; MINERALS; NEISSERIA gonorrhoeae; IRON ions
- Publication
Nature Structural Biology, 2003, Vol 10, Issue 4, p297
- ISSN
1072-8368
- Publication type
Article
- DOI
10.1038/nsb903