We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
A novel in vitro propagation system for West Indian elm [Guazuma ulmifolia Lam. (Malvaceae)]: a valuable medicinal woody species.
- Authors
de Jesus Santana, Mayla; Barbosa-Júnior, Sebastião Martins; Dias, Lana Laene Lima; Silva, Lázara Aline Simões; da Silva, Givanildo Zildo; Fortini, Evandro Alexandre; Batista, Diego Silva; Otoni, Wagner Campos; da Costa Netto, Antônio Paulino; Rocha, Diego Ismael
- Abstract
The aim of this study was to establish a system of in vitro germination and propagation of Guazuma ulmifolia Lam. microcuttings (Malvaceae). Seeds were inoculated into test tubes containing different concentrations (half-strength (½) and full-strength) of Murashige and Skoog (MS) basal salts and sucrose (0, 10, 20, 40, and 80 g L−1). For the induction of multiple shoots, microcuttings were cultured on MS medium supplemented with 0.5, 1.0, and 2.0 mg L−1 of 6-benzyladenine (BA), kinetin (KIN), or thidiazuron (TDZ), or containing no plant growth regulator (control). Nodal segments were also cultured in vessels with two different sealing systems, for example, sealed with a porous membrane (1 M) (21 μL L−1 s−1 of CO2) or with no membrane (0 M) (14 μL L−1 s−1 of CO2). Treatments with full-strength MS salts supplemented with 20% sucrose and ½ MS with 10% sucrose showed 100% germination, whereas there was no germination in the culture media with the highest sucrose concentration (80 g L−1), regardless of the MS salt concentration. The presence of cytokinins in the culture medium reduced shoot length and number of leaves and roots in the microcuttings. Plants cultured in vessels sealed with a porous membrane showed greater total and shoot lengths and greater leaf area. Anatomical analyses also showed that the mesophyll and leaf blade were thicker in plants cultured under higher gas exchange (1 M), a condition that also favored ex vitro plant acclimatization. These results provide relevant information for the establishment of G. ulmifolia micropropagation systems.
- Subjects
PLANT regulators; MALVACEAE; LEAF area; GAS exchange in plants; THIDIAZURON; ACCLIMATIZATION (Plants); WOODY plants; SUCROSE
- Publication
In Vitro Cellular & Developmental Biology Plant, 2022, Vol 58, Issue 6, p865
- ISSN
1054-5476
- Publication type
Article
- DOI
10.1007/s11627-022-10275-8