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- Title
Cloning and characterization of human complement component C7 promoter.
- Authors
González, S; Martínez-Borra, J; López-Larrea, C
- Abstract
To study the transcriptional regulation of the human complement component C7, a 1 kb promoter fragment was cloned and the transcription start site was determined. C7 is expressed by the hepatoma-derived cell line Hep-3B, but not by Hep-G2. Transfection of these cell lines with different C7 promoter -- luciferase constructs demonstrated that 1 kb of the 5'-flanking region contains the necessary elements for driving C7 transcription in a tissue-specific manner and showed that the sequence between -29/+102 retained the majority of C7 promoter activity in Hep-3B. Electrophoretic mobility shift assays suggested that the binding of the C/EBPα transcription factor to a C/EBP sequence located at +42 is essential for C7 expression. To investigate whether the absence of C/EBPα expression in Hep-G2 cells is responsible for the lack of C7 transcription, Hep-G2 cells were transfected with a C/EBPα expression vector. C/EBPα transactivated the C7 luciferase reported gene and restored the C7 expression in Hep-G2 cells.
- Subjects
MOLECULAR cloning; HEPATOCELLULAR carcinoma; ELECTROPHORESIS
- Publication
Genes & Immunity, 2003, Vol 4, Issue 1, p54
- ISSN
1466-4879
- Publication type
Article