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- Title
DNA Polymorphism of Percidae population structure in the Kuibyshev water reservoir of Russia.
- Authors
Islam, A.; Gorshkov, O.V.; Chernov, V.M.; Kuznetsov, V.A.
- Abstract
The pike perchesStizostedion luciopercaandS. volgense, river perchPerca fluviatilisand ruffeGymnocephalus cernuusare important components of the fish fauna of the Kuibyshev reservoir in Russia. We analysed genetic variation within and among populations of these species. Two microsatellite DNA from walleyeS. vitreumwere used as primers of this study. Each of the DNA markers resolved 6–10 alleles with locusSvi4andSvi6and size range of 102–169 base pairs(bp). PCR was performed with the selected primers, with temperature cycles followed a denaturing profile for 30 s at 94° C. A final 10 min elongation step was resumed at 72° C after 35 cycles.Stizostedion volgenseshowed the highest bp of 100 to 3000, differing by sex, age and maturity level. The populations of P. fluviatilis have variable DNA polymorphism within the range 90–1000 base pairs. We also detected a new band in the fingerprints. The degree of neutral genetic variation detected indicates that effective population sizes of the study species are large.
- Subjects
RUSSIA; GENETIC polymorphisms; PERCIDAE; FISH populations; KUIBYSHEV Reservoir (Russia); GENETIC markers; PIKE fisheries; ZANDER; EUROPEAN perch; MICROSATELLITE repeats
- Publication
Journal of Fish Biology, 2004, Vol 65, p330
- ISSN
0022-1112
- Publication type
Article
- DOI
10.1111/j.1095-8649.2004.559al.x