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- Title
First Report of Colistin-Resistant Escherichia coli Carrying mcr-1 IncI2(delta) and IncX4 Plasmids from Camels (Camelus dromedarius) in the Gulf Region.
- Authors
Ghazawi, Akela; Strepis, Nikolaos; Anes, Febin; Yaaqeib, Dana; Ahmed, Amal; AlHosani, Aysha; AlShehhi, Mirah; Manzoor, Ashrat; Habib, Ihab; Wani, Nisar A.; Hays, John P.; Khan, Mushtaq
- Abstract
Addressing the emergence of antimicrobial resistance (AMR) poses a significant challenge in veterinary and public health. In this study, we focused on determining the presence, phenotypic background, and genetic epidemiology of plasmid-mediated colistin resistance (mcr) in Escherichia coli bacteria isolated from camels farmed in the United Arab Emirates (UAE). Fecal samples were collected from 50 camels at a Dubai-based farm in the UAE and colistin-resistant Gram-negative bacilli were isolated using selective culture. Subsequently, a multiplex PCR targeting a range of mcr-genes, plasmid profiling, and whole-genome sequencing (WGS) were conducted. Eleven of fifty camel fecal samples (22%) yielded colonies positive for E. coli isolates carrying the mcr-1 gene on mobile genetic elements. No other mcr-gene variants and no chromosomally located colistin resistance genes were detected. Following plasmid profiling and WGS, nine E. coli isolates from eight camels were selected for in-depth analysis. E. coli sequence types (STs) identified included ST7, ST21, ST24, ST399, ST649, ST999, and STdaa2. Seven IncI2(delta) and two IncX4 plasmids were found to be associated with mcr-1 carriage in these isolates. These findings represent the first identification of mcr-1-carrying plasmids associated with camels in the Gulf region. The presence of mcr-1 in camels from this region was previously unreported and serves as a novel finding in the field of AMR surveillance.
- Subjects
UNITED Arab Emirates; PERSIAN Gulf; CAMELS; CAMEL milk; MOBILE genetic elements; ESCHERICHIA coli; PLASMIDS; WHOLE genome sequencing
- Publication
Antibiotics (2079-6382), 2024, Vol 13, Issue 3, p227
- ISSN
2079-6382
- Publication type
Article
- DOI
10.3390/antibiotics13030227