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- Title
Release of exosomes from injectable silk fibroin and alginate composite hydrogel for treatment of myocardial infarction.
- Authors
Ni, Yunjie; Hua, Yinjian; He, Zhengfei; Hu, Weilv; Chen, Zhiyun; Wang, Diqing; Li, Xintong; Sun, Yanfang; Jiang, Guohua
- Abstract
Myocardial infarction (MI) is considered as a significant cause of death globally. Exosomes (EXOs) are essential for intercellular communication and pathophysiology of several cardiovascular diseases. Nevertheless, the short half-life and rapid clearance of EXOs leads to a lack of therapeutic doses delivered to the lesioned area. Therefore, an injectable silk fibroin and alginate (SF/Alg) composite hydrogel was developed to bind folate receptor-targeted EXOs (FA-EXOs) derived from H9C2 cells for the therapy of myocardial injury following myocardial infarction-ischemia/reperfusion (MI-I/R). The resulting composite exhibits a variety of properties, including adjustable gelation kinetics, shear-thinning injectability, soft and dynamic stability that adapts to the heartbeat, and outstanding cytocompatibility. After injected into the damaged rat heart, administration of SF/Alg + FA-EXOs significantly enhanced cardiac function as demonstrated by improved ejection fraction, fractional shortening and decreased fibrosis area. The results of real-time PCR and immunofluorescence staining show a remarkable up-regulation in the expression of proteins (CD31) and genes (VWF and Serca2a) related to the heart. Conversely, expression of fibrosis-related genes (TGF-β1) decreased significantly. Therefore, the obtained SF/Alg + FA-EXOs system remarkably enhanced the intercellular interactions, promoted cell proliferation and angiogenesis, and achieved an outstanding therapeutic effect on MI.
- Subjects
MYOCARDIAL infarction; REPERFUSION; SILK fibroin; EXOSOMES; ALGINIC acid; HYDROGELS
- Publication
Journal of Biomaterials Applications, 2024, Vol 39, Issue 2, p139
- ISSN
0885-3282
- Publication type
Article
- DOI
10.1177/08853282241251610