We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Examining Caries Risk With the Caracteristic of HLA-DRB1 Gene.
- Authors
Soesilawati, Pratiwi; Notopuro, Harianto; Firdauzy, Muhammad Alwino Bayu; Prahandita, Inara Putri
- Abstract
Introduction: HLA-DRB1 alleles were derived from MHC class II molecules. These alleles encoded sIgA secretion which contribute as a barrier to S. mutans colonization. HLA-DRB1 was known as genes with high mutations causing differences in peptide bond, thus affecting the progression and vulnerability to a disease. The purpose of this study was to determine the effect of mutations in HLA-DRB1 alleles in different dental caries risk. Methods: In this study, we extracted the genomic DNA from whole blood samples of 30 patients with low level dental caries (indeks def-t < 2) as control group and high level dental caries (deft > 3) as case group. HLA-DRB1 varians were studied through genomic DNA isolation for PCR-RFLP. RFLP is analyzed through BseRI, BsaJI, RsaI, and Sau961 restriction enzymes was used in this assay. Results: The PCR-RFLP typing method was evaluated on 60 genomic DNA samples, result found that all samples were divided into 5 groups of variants, two variants in the control group and three variants in the case group. Conclusion: PCR-RFLP was shown to be a sensitive method for the detection of mutation in HLADRB1 alleles caused a dental caries level differences. HLA-DRB mutations caused changes in signal transduction and therefore contributes to imunogenetik pathway of dental caries.
- Subjects
DENTAL caries; GENETIC mutation; PEPTIDE bonds; CELLULAR signal transduction; GENES; DISEASE progression
- Publication
Malaysian Journal of Medicine & Health Sciences, 2020, Vol 16, Issue Suppl4, p67
- ISSN
1675-8544
- Publication type
Article