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- Title
Novel ELISA systems for antibodies to desmoglein 1 and 3: correlation of disease activity with serum autoantibody levels in individual pemphigus patients.
- Authors
Schmidt, Enno; Dähnrich, Cornelia; Rosemann, Anke; Probst, Christian; Komorowski, Lars; Saschenbrecker, Sandra; Schlumberger, Wolfgang; Stöcker, Winfried; Hashimoto, Takashi; Bröcker, Eva-Bettina; Recke, Andreas; Rose, Christian; Zillikens, Detlef
- Abstract
Please cite this paper as: Novel ELISA systems for antibodies to desmoglein 1 and 3: correlation of disease activity with serum autoantibody levels in individual pemphigus patients. Experimental Dermatology 2010; 19: 458–463. Pemphigus vulgaris (PV) and pemphigus foliaceus (PF) are intraepidermal blistering skin diseases. PV is characterised by autoantibodies directed against desmoglein (Dsg) 3 and in patients with the mucocutaneous variant also against Dsg 1, whereas in PF, only Dsg 1 is targeted. Here, ectodomains of Dsg 3 and Dsg 1 were recombinantly expressed in a human cell line (HEK293) and applied as authentic solid phases in ELISA test systems. Autoantibodies against Dsg 3 and/or Dsg 1 could be detected in 71 (100%) of 71 PV sera and against Dsg 1 in 48 (96%) of 50 PF sera. Control sera showed reactivity with Dsg 3 and Dsg 1 in 0.2% and 0.7%, respectively, of 401 healthy blood donors and in 2.1% of 48 randomly selected patients with bullous pemphigoid. No reactivity with Dsg 1 and 3 was detected in 21 patients with linear IgA disease. For both pemphigus variants, a statistically significant correlation between clinical severity and autoantibody levels was observed as demonstrated for 10 PV and 5 PF patients. In conclusion, the use of the ectodomains of Dsg 3 and 1 as target antigens expressed in a human cell line resulted in sensitive and specific ELISA systems for both diagnosis and monitoring of PV and PF.
- Subjects
IMMUNOGLOBULINS; AUTOANTIBODIES; PEMPHIGUS; CELL lines; ENZYME-linked immunosorbent assay
- Publication
Experimental Dermatology, 2010, Vol 19, Issue 5, p458
- ISSN
0906-6705
- Publication type
Article
- DOI
10.1111/j.1600-0625.2010.01069.x