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- Title
Fibulin-5 accelerates elastic fibre assembly in human skin fibroblasts.
- Authors
Katsuta, Yuji; Ogura, Yuki; Iriyama, Shunsuke; Goetinck, Paul F.; Klement, John F.; Uitto, Jouni; Amano, Satoshi
- Abstract
Fibulin-5 null mice display abnormalities in the elastic fibres in the dermis. We postulated, therefore, that fibulin-5 might be a regulator of elastic fibre assembly and stability. To clarify the role of fibulin-5 in elastic fibre formation, we employed in vitro systems that allowed increasing expression of elastic fibre components by gene transduction using retroviral vector constructs. First, the human tropoelastin gene (ELN) was transduced into human dermal fibroblasts, which resulted in elevated gene expression. These cells were then cultured in monolayer, but the overexpression of ELN in this system did not alter the assembly of elastic fibres. However, incubation of fibroblasts with TGF-β1 resulted in elastic fibre accumulation, and the expression of fibulin-5 was enhanced by TGF-β1. Thus, we overexpressed human fibulin-5 cDNA in dermal fibroblasts using a retroviral vector containing a cytomegalovirus (CMV) promoter. These cells deposited elastic fibres. These results suggest that fibulin-5 is a critical component in the control of elastic fibre assembly by dermal fibroblasts.
- Subjects
FIBROBLASTS; DERMIS; ELASTICITY; GENE expression
- Publication
Experimental Dermatology, 2008, Vol 17, Issue 10, p837
- ISSN
0906-6705
- Publication type
Article
- DOI
10.1111/j.1600-0625.2008.00709.x