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- Title
The Arabidopsis heat-intolerant 5 (hit5)/enhanced response to aba 1 (era1) mutant reveals the crucial role of protein farnesylation in plant responses to heat stress.
- Authors
Wu, Jia‐Rong; Wang, Lian‐Chin; Lin, Yu‐Ru; Weng, Chi‐Pei; Yeh, Ching‐Hui; Wu, Shaw‐Jye
- Abstract
• Protein farnesylation is a post-translational modification known to regulate abscisic acid (ABA)-mediated drought tolerance in plants. However, it is unclear whether and to what extent protein farnesylation affects plant tolerance to high-temperature conditions. • The Arabidopsis heat-intolerant 5 (hit5) mutant was isolated because it was thermosensitive to prolonged heat incubation at 37°C for 4 d but thermotolerant to sudden heat shock at 44°C for 40 min. Map-based cloning revealed that HIT5 encodes the β-subunit of the protein farnesyltransferase. hit5 was crossed with the aba-insensitive 3 (abi3) mutant, the aba-deficient 3 (aba3) mutant, and the heat shock protein 101 (hsp101) mutant, to characterize the HIT5-mediated heat stress response. • hit5/abi3 and hit5/aba3 double mutants had the same temperature-dependent phenotypes as hit5. Additionally, exogenous supplementation of neither ABA nor the ABA synthesis inhibitor fluridone altered the temperature-dependent phenotypes of hit5. The hit5/hsp101double mutant was still sensitive to prolonged heat incubation, yet its ability to tolerate sudden heat shock was lost. • The results suggest that protein farnesylation either positively or negatively affects the ability of plants to survive heat stress, depending on the intensity and duration of high-temperature exposure, in an ABA-independent manner. HSP101 is involved in the hit5-derived heat shock tolerance phenotype.
- Subjects
ARABIDOPSIS; BRASSICACEAE; ARABIDOPSIS arenosa; ALLIARIA
- Publication
New Phytologist, 2017, Vol 213, Issue 3, p1181
- ISSN
0028-646X
- Publication type
Article
- DOI
10.1111/nph.14212