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- Title
cDNA cloning of a human RAB26-related gene encoding a Ras-like GTP-binding protein on chromosome 16p13.3 region.
- Authors
Seki, N.; Yoshikawa, T.; Hattori, A.; Miyajima, N.; Muramatsu, M.; Saito, T.
- Abstract
Abstract Members of the RAB protein family are important regulators of vesicular fusion and trafficking. A putative new member of the RAB family of genes was identified through a public database search, and its full-length cDNA was isolated from a human fetal brain cDNA library. The predicted protein product of the gene consists of 190 amino acid residues and has 87% identity with rat Rab26. Thus, we designated this gene as the human RAB26-related gene. Reverse transcription-coupled polymerase chain reaction (RT-PCR) demonstrated that the RAB26-related messenger RNA was predominantly expressed in adult and fetal brain. Furthermore, an RT-PCR experiment for brain subregions showed that the mRNA was highly expressed in the amygdala, cerebellum, caudate nucleus, and hippocampus. By PCR-based analysis with both a human/rodent monochromosomal hybrid cell panel and a radiation hybrid panel, the gene was mapped to the chromosome 16p13.3 region between markers W1-7742 and WI-3061. The RAB26-related gene consists of eight exons that span about 44kb of the genome DNA.
- Subjects
GENES; FETAL brain; AMINO acids; POLYMERASE chain reaction; AMYGDALOID body
- Publication
Journal of Human Genetics, 2000, Vol 45, Issue 5, p309
- ISSN
1434-5161
- Publication type
Article
- DOI
10.1007/s100380070023