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- Title
Matrix Protein 2 Vaccination and Protection against Influenza Viruses, Including Subtype H5N1.
- Authors
Tompkins, Stephen Mark; Zi-Shan Zhao; Chia-Yunb Lo; Misplon, Julia A.; Liu, Teresa; Zhiping Ye; Hogan, Robert J.; Zhengqi Wu; Benton, Kimberly A.; Tumpey, Terrence M.; Epstein, Suzanne L.
- Abstract
Changes in influenza viruses require regular reformulation of strain-specific influenza vaccines. Vaccines based on conserved antigens provide broader protection. Influenza matrix protein 2 (M2) is highly conserved across influenza A subtypes. To evaluate its efficacy as a vaccine candidate, we vaccinated mice with M2 peptide of a widely shared consensus sequence. This vaccination induced antibodies that cross-reacted with divergent M2 peptide from an H5N1 subtype. A DNA vaccine expressing full-length consensus-sequence M2 (M2-DNA) induced M2-specific antibody responses and protected against challenge with lethal influenza. Mice primed with M2-DNA and then boosted with recombinant adenovirus expressing M2 (M2-Ad) had enhanced antibody responses that cross-reacted with human and avian M2 sequences and produced T-cell responses. This M2 prime-boost vaccination conferred broad protection against challenge with lethal influenza A, including an H5N1 strain. Vaccination with M2, with key sequences represented, may provide broad protection against influenza A.
- Subjects
VACCINATION; EXTRACELLULAR matrix proteins; INFLUENZA A virus; INFLUENZA viruses; PEPTIDES; IMMUNOGLOBULINS; ADENOVIRUSES; T cells
- Publication
Emerging Infectious Diseases, 2007, Vol 13, Issue 3, p426
- ISSN
1080-6040
- Publication type
Article
- DOI
10.3201/eid1303.061125