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- Title
Artocarpus communis Induces Autophagic Instead of Apoptotic Cell Death in Human Hepatocellular Carcinoma Cells.
- Authors
Tzeng, Cheng-Wei; Tzeng, Wen-Sheng; Lin, Liang-Tzung; Lee, Chiang-Wen; Yen, Ming-Hong; Yen, Feng-Lin; Lin, Chun-Ching
- Abstract
For centuries, natural plant extracts have played an important role in traditional medicine for curing and preventing diseases. Studies have revealed that Artocarpus communis possess various bioactivities, such as anti-inflammation, anti-oxidant, and anticancer activities. A. communis offers economic value as a source of edible fruit, yields timber, and is widely used in folk medicines. However, little is known about its molecular mechanisms of anticancer activity. Here, we demonstrate the antiproliferative activity of A. communis methanol extract (AM) and its dichloromethane fraction (AD) in two human hepatocellular carcinoma (HCC) cell lines, HepG2 and PLC/PRF/5. Colony assay showed the long-term inhibitory effect of both extracts on cell growth. DNA laddering and immunoblotting analyses revealed that both extracts did not induce apoptosis in the hepatoma cell lines. AM and AD-treated cells demonstrated different cell cycle distribution compared to UV-treated cells, which presented apoptotic cell death with high sub-G1 ratio. Instead, acridine orange staining revealed that AM and AD triggered autophagosome accumulation. Immunoblotting showed a significant expression of autophagy-related proteins, which indicated the autophagic cell death (ACD) of hepatoma cell lines. This study therefore demonstrates that A. communis AM and its dichloromethane fraction can induce ACD in HCC cells and elucidates the potential of A. communis extracts for development as anti tumor therapeutic agents that utilize autophagy as mechanism in mediating cancer cell death.
- Subjects
PLANT protein analysis; AUTOPHAGY; ANTINEOPLASTIC agents; APOPTOSIS; BIOLOGICAL assay; CELL culture; CELL cycle; CELL lines; FLOW cytometry; HEPATOCELLULAR carcinoma; HIGH performance liquid chromatography; PLANT proteins; RESEARCH funding; STATISTICS; WESTERN immunoblotting; PLANT extracts; DATA analysis; DATA analysis software; DESCRIPTIVE statistics; ONE-way analysis of variance
- Publication
American Journal of Chinese Medicine, 2015, Vol 43, Issue 3, p559
- ISSN
0192-415X
- Publication type
Article
- DOI
10.1142/S0192415X15500354