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- Title
Nonsterilized fermentative production of poly‐γ‐glutamic acid from cassava starch and corn steep powder by a thermophilic Bacillus subtilis.
- Authors
Zeng, Wei; Chen, Guiguang; Wu, Yange; Dong, Mengna; Zhang, Bin; Liang, Zhiqun
- Abstract
Abstract: BACKGROUND: Poly‐γ‐glutamic acid (γ‐PGA) is a homopolymer obtained by microbial fermentation, which has wide potential applications in many fields. However, costs of raw materials and the fermentation process are still a limiting factor in the commercial‐scale production of γ‐PGA. Thus, it is necessary to continue to optimize its production by developing a cost‐effective fermentation process. RESULTS: Bacillus subtilis GXC‐36 was proven to be able to produce γ‐PGA efficiently by nonsterilized fermentation. The yield of γ‐PGA reached 36.38 ± 0.81 g L−1 with a high productivity of 1.65 g L−1 h−1 by nonsterilized batch fermentation in a 10 L fermenter using glucose and yeast extract (YE) as carbon and nitrogen source. Furthermore, B. subtilis GXC‐36 could produce γ‐PGA efficiently using cassava starch (CS) and corn steep powder (CSP) instead of glucose and YE for γ‐PGA production, and the yield of γ‐PGA also reached 33.51 ± 0.72 g L−1 with productivity of 1.52 g L−1 h−1 in a 10 L fermenter. Fermentative benefits evaluation indicated that the fermentation cost of nonsterilized fermentation using CS and CSP was only 60.6% of sterilized fermentation using glucose and YE. CONCLUSION: This study provides an economical and simple process for γ‐PGA production by nonsterilized fermentation with low‐cost raw materials. © 2018 Society of Chemical Industry
- Subjects
GLUTAMIC acid; CASSAVA starch; THERMOPHILIC bacteria; BACILLUS subtilis; FERMENTATION; YEAST extract
- Publication
Journal of Chemical Technology & Biotechnology, 2018, Vol 93, Issue 10, p2917
- ISSN
0268-2575
- Publication type
Article
- DOI
10.1002/jctb.5646