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- Title
Spatial computation of intratumoral T cells correlates with survival of patients with pancreatic cancer.
- Authors
Carstens, Julienne L.; Correa de Sampaio, Pedro; Yang, Dalu; Barua, Souptik; Wang, Huamin; Rao, Arvind; Allison, James P.; LeBleu, Valerie S.; Kalluri, Raghu
- Abstract
The exact nature and dynamics of pancreatic ductal adenocarcinoma (PDAC) immune composition remains largely unknown. Desmoplasia is suggested to polarize PDAC immunity. Therefore, a comprehensive evaluation of the composition and distribution of desmoplastic elements and T-cell infiltration is necessary to delineate their roles. Here we develop a novel computational imaging technology for the simultaneous evaluation of eight distinct markers, allowing for spatial analysis of distinct populations within the same section. We report a heterogeneous population of infiltrating T lymphocytes. Spatial distribution of cytotoxic T cells in proximity to cancer cells correlates with increased overall patient survival. Collagen-I and αSMA+ fibroblasts do not correlate with paucity in T-cell accumulation, suggesting that PDAC desmoplasia may not be a simple physical barrier. Further exploration of this technology may improve our understanding of how specific stromal composition could impact T-cell activity, with potential impact on the optimization of immune-modulatory therapies.
- Publication
Nature Communications, 2017, Vol 8, Issue 4, p15095
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/ncomms15095