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- Title
Analysis of large deletion mutations induced by abasic site analog in human cells.
- Authors
Suzuki, Tetsuya; Katayama, Yuri; Komatsu, Yasuo; Kamiya, Hiroyuki
- Abstract
Background: Abasic sites are formed spontaneously and by nucleobase chemical modifications and base excision repair. A chemically stable abasic site analog was site-specifically introduced into replicable plasmid DNAs, which were transfected into human U2OS cells. The amplified DNAs were recovered from the cells and used for the transformation of a bacterial indicator strain. Results: Large deletion mutations were induced by the analog, in addition to point mutations at the modified site. No apparent sequence homology at the deletion junctions was found. Conclusion: These results suggested that the large deletions induced by the abasic site analog are formed by homology-independent events.
- Subjects
APURINIC acid; DNA; GENETIC mutation
- Publication
Genes & Environment, 2018, Vol 40, Issue 1, pN.PAG
- ISSN
1880-7046
- Publication type
Article
- DOI
10.1186/s41021-018-0110-7