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- Title
Intragenic hypomethylation of <italic>DNMT3A</italic> in patients with myelodysplastic syndrome.
- Authors
Zhang, Ying-Ying; Zhou, Jing-Dong; Yang, Dong-Qin; He, Pin-Fang; Yao, Dong-Ming; Qian, Zhen; Yang, Jing; Xu, Wen-Rong; Lin, Jiang; Qian, Jun
- Abstract
Background: <italic>DNMT3A</italic> is a DNA methyltransferase that acts in <italic>de novo</italic> methylation. Aberrant expression of <italic>DNMT3A</italic> has been reported in several human diseases, including myelodysplastic syndrome (MDS). However, the pattern of <italic>DNMT3A</italic> methylation remains unknown in MDS. Methods: The present study was aimed to investigate the methylation status of <italic>DNMT3A</italic> intragenic differentially methylated region 2 (DMR2) using real-time quantitative methylation-specific PCR and analyze its clinical significance in MDS. Results: Aberrant hypomethylation of <italic>DNMT3A</italic> was found in 57% (51/90) MDS cases. There were no significant differences in age, sex, white blood cell counts, platelet counts, hemoglobin counts and World Health Organization, International Prognostic Scoring System and karyotype classifications between <italic>DNMT3A</italic> hypomethylated and <italic>DNMT3A</italic> hypermethylated groups. However, the patients with <italic>DNMT3A</italic> hypomethylation had shorter overall survival time than those without <italic>DNMT3A</italic> hypomethylation (11 months vs. 36 months, p=0.033). Multivariate analysis confirmed the independent adverse impact of <italic>DNMT3A</italic> hypomethylation in MDS. Conclusions: Our data suggest that <italic>DNMT3A</italic> DMR2 hypomethylation may be a negative prognostic hallmark in MDS.
- Subjects
MYELODYSPLASTIC syndromes; DNA methyltransferases; DNA methylation; POLYMERASE chain reaction; KARYOTYPES
- Publication
Clinical Chemistry & Laboratory Medicine, 2018, Vol 56, Issue 3, p485
- ISSN
1434-6621
- Publication type
Article
- DOI
10.1515/cclm-2016-0142