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- Title
The Diversity of Prokaryotic DDE Transposases of the Mutator Superfamily, Insertion Specificity, and Association with Conjugation Machineries.
- Authors
Guérillot, Romain; Siguier, Patricia; Gourbeyre, Edith; Chandler, Michael; Glaser, Philippe
- Abstract
Transposable elements (TEs) are major components of both prokaryotic and eukaryotic genomes and play a significant role in their evolution. In this study, we have identified new prokaryotic DDE transposase families related to the eukaryoticMutator-like transposases. These genes were retrieved by cascade PSI-Blast using as initial query the transposase of the streptococcal integrative and conjugative element (ICE) TnGBS2. By combiningsecondary structure predictions andprotein sequence alignments,wepredicted the DDE catalytic triad and the DNA-binding domain recognizing the terminal inverted repeats. Furthermore, we systematically characterized the organization and the insertion specificity of the TEs relying on these prokaryoticMutator-like transposases (p-MULT) for theirmobility. Strikingly,twodistant TE families target their integration upstream σA dependentpromoters. This allowedus toidentify a transposase sequence signature associated with this unique insertion specificity and to showthat the dissymmetry between the two inverted repeats is responsible for the orientation of the insertion. Surprisingly, while DDE transposases are generally associated with small and simple transposons such as insertion sequences (ISs), p-MULT encoding TEs show an unprecedented diversity with several families of IS, transposons, and ICEs ranging in size from 1.1 to 52 kb.
- Subjects
TRANSPOSONS; EUKARYOTIC genomes; DDE (Pesticide); DYNAMIC data exchange; HEREDITY; AMINO acid sequence; EUKARYOTIC cells
- Publication
Genome Biology & Evolution, 2014, Vol 6, Issue 2, p260
- ISSN
1759-6653
- Publication type
Article
- DOI
10.1093/gbe/evu010