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- Title
Development of a proteoliposome model to probe transmembrane electron-transfer reactions.
- Authors
White, Gaye F.; Zhi Shi; Liang Shi; Dohnalkova, Alice C.; Fredrickson, James K.; Zachara, John M.; Butt, Julea N.; Richardson, David J.; Clarke, Thomas A.
- Abstract
The mineral-respiring bacterium Shewanella oneidensis uses a protein complex, MtrCAB, composed of two decahaem cytochromes brought together inside a transmembrane porin to transport electrons across the outer membrane to a variety of mineral-based electron acceptors. A proteoliposome system has been developed that contains Methyl Viologen as an internalized electron carrier and valinomycin as a membraneassociated cation exchanger. These proteoliposomes can be used as a model system to investigate MtrCAB function.
- Subjects
CHARGE exchange; SHEWANELLA oneidensis; CYTOCHROMES; PORINS (Proteins); MEMBRANE proteins
- Publication
Biochemical Society Transactions, 2012, Vol 40, Issue 6, p1257
- ISSN
0300-5127
- Publication type
Article
- DOI
10.1042/BST20120116