We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Study on a Luminol-based Electrochemiluminescent Sensor for Label-Free DNA Sensing.
- Authors
Hai-Hong Chu; Ji-Lin Yan; Yi-Feng Tu
- Abstract
Automatic, inexpensive, simple and sensitive methods for DNA sensing and quantification are highly desirable for biomedical research. The rapid development of both the fundamentals and applications of electrochemiluminescence (ECL) over the past years has demonstrated its potential for analytical and bio-analytical chemistry. This paper reports the quenching effect of DNA on the ECL of luminol and the further development of a DNA sensing device. With the pre-functionalization by a composite of carbon nano-tubes (CNTs) and Au nanoparticles (AuNPs), the sensor provides a novel and valuable label-free approach for DNA sensing. Here the ECL intensity was remarkably decreased when more than 1.0 × 10-12 molar of DNA were adsorbed on the sensor. Linearity of the DNA amount with the reciprocal of ECL intensity was observed. A saturated sensor caused a 92.8% quenching effect. The research also proposes the mechanism for the quenching effect which could be attributed to the interaction between luminol and DNA and the elimination of reactive oxygen species (ROSs) by DNA.
- Subjects
NANOPARTICLES; CARBON nanotubes; DETECTORS; REACTIVE oxygen species; MEDICAL sciences
- Publication
Sensors (14248220), 2010, Vol 10, Issue 10, p9481
- ISSN
1424-8220
- Publication type
Article
- DOI
10.3390/s101009481