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- Title
Comparison of L- and D-Amino Acids for Bacterial Imaging in Lung Infection Mouse Model.
- Authors
Muranaka, Yuka; Mizutani, Asuka; Kobayashi, Masato; Nakamoto, Koya; Matsue, Miki; Nishi, Kodai; Yamazaki, Kana; Nishii, Ryuichi; Shikano, Naoto; Okamoto, Shigefumi; Kawai, Keiichi
- Abstract
The effectiveness of L- and D-amino acids for detecting the early stage of infection in bacterial imaging was compared. We evaluated the accumulation of 3H-L-methionine (Met), 3H-D-Met, 3H-L-alanine (Ala), and 3H-D-Ala in E. coli EC-14 and HaCaT cells. Biological distribution was assessed in control and lung-infection-model mice with EC-14 using 3H-L- and D-Met, and 18F-FDG. A maximum accumulation of 3H-L- and D-Met, and 3H-L- and D-Ala occurred in the growth phase of EC-14 in vitro. The accumulation of 3H-L-Met and L-Ala was greater than that of 3H-D-Met and D-Ala in both EC-14 and HaCaT cells. For all radiotracers, the accumulation was greater in EC-14 than in HaCaT cells at early time points. The accumulation was identified at 5 min after injection in EC-14, whereas the accumulation gradually increased in HaCaT cells over time. There was little difference in biodistribution between 3H-L-and D-Met except in the brain. 3H-L- and D-Met were sensitive for detecting areas of infection after the spread of bacteria throughout the body, whereas 18F-FDG mainly detected primary infection areas. Therefore, 11C-L- and D-Met, radioisotopes that differ only in terms of 3H labeling, could be superior to 18F-FDG for detecting bacterial infection in lung-infection-model mice.
- Subjects
LUNG infections; LABORATORY mice; ANIMAL disease models; BACTERIAL diseases; INFECTION
- Publication
International Journal of Molecular Sciences, 2022, Vol 23, Issue 5, p2467
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms23052467