We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Glucose-Dependent Insulinotropic Polypeptide Mitigates 6-OHDA-Induced Behavioral Impairments in Parkinsonian Rats.
- Authors
Yu-Wen Yu; Shih-Chang Hsueh; Jing-Huei Lai; Yen-Hua Chen; Shuo-Jhen Kang; Kai-Yun Chen; Tsung-Hsun Hsieh; Hoffer, Barry J.; Yazhou Li; Greig, Nigel H.; Yung-Hsiao Chiang
- Abstract
In the present study, the effectiveness of glucose-dependent insulinotropic polypeptide (GIP) was evaluated by behavioral tests in 6-hydroxydopamine (6-OHDA) hemi-parkinsonian (PD) rats. Pharmacokinetic measurements of GIP were carried out at the same dose studied behaviorally, as well as at a lower dose used previously. GIP was delivered by subcutaneous administration (s.c.) using implanted ALZET micro-osmotic pumps. After two days of pre-treatment, male Sprague Dawley rats received a single unilateral injection of 6-OHDA into the medial forebrain bundle (MFB). The neuroprotective effects of GIP were evaluated by apomorphine-induced contralateral rotations, as well as by locomotor and anxiety-like behaviors in open-field tests. Concentrations of human active and total GIP were measured in plasma during a five-day treatment period by ELISA and were found to be within a clinically translatable range. GIP pretreatment reduced behavioral abnormalities induced by the unilateral nigrostriatal dopamine (DA) lesion produced by 6-OHDA, and thus may be a novel target for PD therapeutic development.
- Subjects
POLYPEPTIDES; PHARMACOKINETICS; ENZYME-linked immunosorbent assay; APOMORPHINE; DOPAMINE
- Publication
International Journal of Molecular Sciences, 2018, Vol 19, Issue 4, p1153
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms19041153