We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Rolling Adhesion Kinematics of Yeast Engineered To Express Selectins.
- Authors
Bhatia; S. K.; Swers; J. S.; Camphausen; R. T.; Wittrup; K. D.; Hammer; D. A.
- Abstract
Selectins are cell adhesion molecules that mediate capture of leukocytes on vascular endothelium as an essential component of the inflammatory response. Here we describe a method for yeast surface display of selectins, together with a functional assay that measures rolling adhesion of selectin-expressing yeast on a ligand-coated surface. E-selectin-expressing yeast roll specifically on surfaces bearing sialyl-Lewis-x ligands. Observation of yeast rolling dynamics at various stages of their life cycle indicates that the kinematics of yeast motion depends on the ratio of the bud radius to the parent radius (B/P). Large-budded yeast walk across the surface, alternately pivoting about bud and parent. Small-budded yeast wobble across the surface, with bud pivoting about parent. Tracking the bud location of budding yeast allows measurement of the angular velocity of the yeast particle. Comparison of translational and angular velocities of budding yeast demonstrates that selectin-expressing cells are rolling rather than slipping across ligand-coated surfaces.
- Subjects
CELL adhesion; KINEMATICS; YEAST; SELECTINS
- Publication
Biotechnology Progress, 2003, Vol 19, Issue 3, p1033
- ISSN
8756-7938
- Publication type
Article
- DOI
10.1021/bp025756b