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- Title
Effects of endocrine disrupting chemicals on expression of phospholipid hydroperoxide glutathione peroxidase mRNA in rat testes.
- Authors
In-Jeoung Baek; Jung-Min Yon; Se-Ra Lee; Yan Jin; Mi-Ra Kim; Byeongwoo Ahn; Jin Tae Hong; Young-Kug Choo; Beom Jun Lee; Young Won Yun; Sang-Yoon Nam
- Abstract
Phospholipid hydroperoxide glutathione peroxidase (PHGPx), an antioxidative selenoprotein, is modulated by estrogen in the testis and oviduct. To examine whether potential endocrine disrupting chemicals (EDCs) affect the microenvironment of the testes, the expression patterns of PHGPx mRNA and histological changes were analyzed in 5-week-old Sprague-Dawley male rats exposed to several EDCs such as an androgenic compound [testosterone (50,200, and 1,000 μg/kg)], anti-androgenic compounds [flutamide (1, 5, and 25 mg/kg), ketoconazole (0.2 and 1 mg/kg), and diethylhexyl phthalate (10, 50, and 250 mg/kg)], and estrogenic compounds [nonylphenol (10, 50, 100, and 250 mg/kg), octylphenol (10, 50, and 250 mg/kg), and iethylstilbestrol (10, 20, and 40 μg/kg)] daily for 3 weeks via oral administration. Mild proliferation of germ cells and hyperplasia of interstitial cells were observed in the testes of the flutamide-treated group and deletion of the germinal epithelium and sloughing of germ cells were observed in testes of the diethylstilbestrol-treated group. Treatment with testosterone was shown to slightly decrease PHGPx mRNA levels in testes by the reverse transcriptionpolymerase chain reaction. However, anti-androgenic compounds (flutamide, ketoconazole, and diethylhexyl phthalate) and estrogenic compounds (nonylphenol, octylphenol, and diethylstilbestrol) significantly upregulated PHGPx mRNA in the testes (p < 0.05). These findings indicate that the EDCs might have a detrimental effect on spermatogenesis via abnormal enhancement of PHGPx expression in testes and that PHGPx is useful as a biomarker for toxicity screening of estrogenic or antiandrogenic EDCs in testes.
- Subjects
SELENOPROTEINS; PHOSPHOLIPIDS; GLUTATHIONE; PEROXIDASE; ESTROGEN; TESTIS; MESSENGER RNA; ANIMAL models in research
- Publication
Journal of Veterinary Science, 2007, Vol 8, Issue 3, p213
- ISSN
1229-845X
- Publication type
Article
- DOI
10.4142/jvs.2007.8.3.213