We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Effects of pulsed electrical stimulation on α-smooth muscle actin and type I collagen expression in human dermal fibroblasts.
- Authors
Urabe, Hiroya; Akimoto, Ryuji; Kamiya, Shohei; Hosoki, Katsu; Ichikawa, Hideyuki; Nishiyama, Toshio
- Abstract
Pulsed electrical stimulation (PES) is known to affect cellular activities. We previously found PES to human dermal fibroblasts (HFs) promoted platelet-derived growth factor subunit A (PDGFA) gene expression, which enhanced proliferation. In this study, we investigated PES effects on fibroblast collagen production and differentiation into myofibroblasts. HFs were electrically stimulated at 4800 Hz and 5 V for 60 min. Imatinib, a specific inhibitor of PDGF receptors, was treated before PES. After 6 h of PES, PDGFA, α-smooth muscle actin (α-SMA), and collagen type I α1 chain gene expressions were upregulated in PES group. Imatinib suppressed the promoted expression except for PDGFA. Immunofluorescence staining and enzyme-linked immunosorbent assay showed the production of α-SMA and collagen I was enhanced in PES group but suppressed in PES + imatinib group at 48 h after PES. Therefore, PES promotes the production of α-SMA and collagen I in fibroblasts, which is triggered by PDGFA that is upregulated early after PES.
- Subjects
ELECTRIC stimulation; GENE expression; PLATELET-derived growth factor; FIBROBLASTS; ACTIN; COLLAGEN
- Publication
Bioscience, Biotechnology & Biochemistry, 2024, Vol 88, Issue 5, p522
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1093/bbb/zbae017